Comparative study of metal induced phospholipid modifications in the heavy metal tolerant filamentous fungus Paecilomyces marquandii and implications for the fungal membrane integrity.

نویسندگان

  • Mirosława Słaba
  • Przemysław Bernat
  • Sylwia Różalska
  • Justyna Nykiel
  • Jerzy Długoński
چکیده

In this work we compared the effect of five heavy metals: Zn, Pb, Cd, Ni and Cu on phospholipid composition of the ubiquitous soil fungus Paecilomyces marquandii, originating from a strongly metal polluted area and characterized by high tolerance to these elements. Cd, Ni and Cu caused an increase in phosphatidylcholine (PC). Only Pb decreased PC content, which was accompanied by a significant rise in the phosphatidic acids (PA) level, probably due to activation of phospholipase D which hydrolyzes PC to PA. This could result in membrane fluidity disturbance, and thus affect its integrity. The assessment of propidium iodide influx showed strong disturbance of membrane integrity for Cu and Pb stressed mycelia, whereas mycelia treated with Ni were impermeable to this dye. The results obtained revealed a strong Cu and Pb toxicity involving disruption of membrane integrity. Pb action was reflected by lipid composition, whereas changes in Cu treated mycelia did not completely elucidate its harmful effect on the membrane, which was most probably caused by Cu induced lipid peroxidation. Zn did not induce quantitative changes in PC and phosphatidylethanolamine (PE) but caused changes in phospholipid lipid saturation, which appears to be important for fungus adaptation to the presence of metals. The enhanced PC content balanced by higher PC saturation can help in the maintenance of proper membrane fluidity and result in alleviating the Cd and Ni induced stress. These results will allow to clarify the mechanism of Pb toxicity and help to elucidate the cellular basis of fungal membrane adaptation to heavy metals.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

First report of Paecilomyces marquandii from Iran

During 2008, the presence of Paecilomyces species was studied in fields in Fars province. Soil samples were collected from 0–20 cm depth. The isolates were recovered using a soil dilution plate method directly from soil. Isolation was performed from soil using malt extract agar, rice agar, oat-meal agar and potato dextrose agar. Anamorphic characteristics such as morphology of conidiophores, ph...

متن کامل

The Effect of Heavy Metal Ions and Their Complexions upon Growth, Sporulation and Pathogenicity of the Entomopathogenic Fungus Paecilomyces farinosus

Heavy metal ions are harmful for entomopathogenic fungi. Derivatives of starch dialdehyde such as dihydrazone (DHZ), dithiosemicarbazone (DTSC), and dioxime (DOX) were used to form water-insoluble complexes with heavy metal ions. This phenomenon thus provided protection of entomopathogenic fungus Paecilomyces farinosus from the toxic effects of heavy metal ions. The fungus growth and the sporul...

متن کامل

Fungus An alternative for bioremediation of heavy metal containing wastewater: A review

The common filamentous fungi can sorb heavy metals (Zn, Cd, Pb, Fe, Ni, Ag, Th, Ra & U) from aqueous solutions to varying extents. Fungal biosorption largely depends on parameters such as pH, metal ion and biomass concentration, physical or chemical pretreatment of biomass, and presence of various legends in solution. When compared with commercial ion exchange resins, carbons and metal oxides, ...

متن کامل

Surface Recognition and Complexations Between Synthetic Poly(ribo)nucleotides and Neutral Phospholipids and Their Implications in Lipofection

Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation or surfactant-induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followe...

متن کامل

Surface Recognition and Complexations Between Synthetic Poly(ribo)nucleotides and Neutral Phospholipids and Their Implications in Lipofection

Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation or surfactant-induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followe...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Acta biochimica Polonica

دوره 60 4  شماره 

صفحات  -

تاریخ انتشار 2013